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KMID : 1094020140310030194
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Journal of Veterinary Clinics
2014 Volume.31 No. 3 p.194 ~ p.198
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Prevalence of Methicillin-resistant Staphylococci Isolates from Horses and Horse-related Personnel in Korea
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Lee Sang-Kyu
Han Jae-Ik
Kim Ill-Hwa
Na Ki-Jeong
Kang Hyun-Gu
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Abstract
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Methicillin-resistant staphylococci (MRS) are emerging as important pathogens in humans and animalsworldwide. The aim of this study was to investigate the prevalence of MRS in the racehorse population and in horserelatedpersonnel in Korea. A total of 195 horses and 18 humans (eight veterinarians, three veterinary hospital staff,and seven horse-handlers) from racehorse farms in Korea were included in the study. The samples were collectedfrom nasal cavities using bacterial transport medium and were cultivated on tryptic soy agar with 5% sheep bloodfor 3 days at 37oC to confirm the presence of Staphylococcus spp. Presumptive Staphylococcus spp. isolates wereidentified by 16S ribosomal RNA gene analysis. The coagulase test and oxacillin susceptibility tests were performedusing the tube dilution and disk diffusion methods, respectively. The presence of the mecA gene was determined usinga polymerase chain reaction assay. Of the 195 horses, 29 (15.6%) yielded 29 MRS isolates. Twelve (66.7%) of the18 horse-related personnel yielded 12 MRS isolates. All of the MRS isolates from horses or horse-related personnelwere identified as methicillin-resistant coagulase-negative staphylococci (MRCNS). The result of this study suggestthat the prevalence of MRS increased with the duration of antibiotic use (p = 0.002). This study also provides evidencefor the zoonotic transmission of MRCNS between horses and humans, although further investigations are needed.
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KEYWORD
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racing horse, methicillin-resistant coagulase-negative staphylococci
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